pbs washes (Servicebio Inc)
86
Structured Review
Servicebio Inc
pbs washes
Pbs Washes, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbs+washes/488+alexa+anti+conjugated+fluor+goat+igg+rabbit/pmc12963903-86-2-7
Average 86 stars, based on 1 article reviews
Pbs Washes, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbs+washes/488+alexa+anti+conjugated+fluor+goat+igg+rabbit/pmc12963903-86-2-7
Average 86 stars, based on 1 article reviews
pbs washes - by Bioz Stars,
2026-09
86/100 stars
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Microscopy:Article Title: Circadian Disruption Through Light–Dark Cycle Alteration Induced Alzheimer’s Disease-like Pathology in Mice Article Snippet: After washing three times with PBS, sections were incubated with HRP-conjugated goat anti-rabbit secondary antibody (1:300; Servicebio, Wuhan, China) for 50 min at 20–24 °C. .. Following four Article Title: Circadian Disruption Through Light-Dark Cycle Alteration Induced Alzheimer's Disease-like Pathology in Mice. Article Snippet: After washing three times with PBS, sections were incubated with HRPconjugated goat anti-rabbit secondary antibody (1:300; Servicebio, Wuhan, China) for 50 min at 20–24 ◦C. .. Following four Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100). .. After three Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection-Driven cartilage regeneration. Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 104 cells per well) and cultured to ~70–80% confluence, stimulated with LPS (10 μg mL− 1, 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 ◦C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100). .. After three Incubation:Article Title: USP38 regulates autophagy-dependent ferroptosis by deubiquitinating CTNNB1 in melanoma. Article Snippet: The incidence of melanoma continues to rise gradually, and its clinical management remains challenging.. Ubiquitin-specific protease 38 (USP38) regulates tumor progression in multiple cancers, but its mechanism in melanoma remains elusive.. We discovered USP38 overexpression in human melanoma tissues. Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100). .. After three Article Title: Therapeutic effects of Danhong injection on ischemic stroke recovery: dual modulation of angiogenesis and neurogenesis via activating tyrosine kinase signaling. Article Snippet: Ethnopharmacological relevance: Danhong injection (DI), a standardized traditional Chinese medicine injection prepared from Salvia miltiorrhiza Bge. and Carthamus tinctorius L., has been validated in clinical studies for its therapeutic efficacy and is widely used in stroke treatment.. However, the exact molecular mechanisms behind its neuroprotective effects during recovery remain unclear.. Aim of the study: This research aims to evaluate DI’s therapeutic potential in promoting functional recovery following cerebral ischemia/reperfusion (CI/R) injury and to uncover the molecular basis of its neuroprotective Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection-Driven cartilage regeneration. Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 104 cells per well) and cultured to ~70–80% confluence, stimulated with LPS (10 μg mL− 1, 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 ◦C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100). .. After three Article Title: Fecal microbiota transplantation ameliorates radiation-induced lung injury by reshaping gut metabolic homeostasis to activate FAM134B-mediated ER-phagy Article Snippet: After blocking with Quick Blocker (Beyotime, China) for 15 minutes, they were incubated overnight at 4°C with specific primary antibodies. .. Following Labeling:Article Title: Fecal microbiota transplantation ameliorates radiation-induced lung injury by reshaping gut metabolic homeostasis to activate FAM134B-mediated ER-phagy Article Snippet: After blocking with Quick Blocker (Beyotime, China) for 15 minutes, they were incubated overnight at 4°C with specific primary antibodies. .. Following |